Correlation Between Staining Degree of Mycelia of Mortierella Alpina by Triphenyltetrazolium Chloride and Arachidonic Acid Content in the Fungal Lipids 高山被孢霉的红四氮唑染色程度与菌体油脂中花生四烯酸含量的关系
Infarct volume was detected by triphenyltetrazolium chloride ( TTC) staining and pathological changes were observed microscopically in HE stained sections. 各组大鼠处死后行TTC和HE染色,测定脑梗死体积并观察病理学改变。
Creatine kinase assay, triphenyltetrazolium chloride staining, and transmission electron microscopy analysis demonstrated more serious myocardial damage in the IR-G group than in the IR+ G group. CK分析、TTC染色和透射电镜分析证明lR-G组比IR+G组有更多的心肌损伤。
The staining degree of inoculum mycelia stained by triphenyltetrazolium chloride increased with the inoculum age, which positively correlated to the lipids content in fungal biomass and arachidonic acid content in the fungal lipids. 高山被孢霉的种子菌体被TTC染色的程度随种龄增加而增加,而种子中的油脂含量和油脂中的花生四烯酸含量也都随种龄增加而增加。
Rat model of cerebral ischemia-reperfusion injury was created by the middle cerebral artery occlusion ( MCAO) with modified suture method, The nervous deficit symptoms were observed and the infarct volume was measured by triphenyltetrazolium chloride ( TTC) staining after the cerebral ischemia. 方法:1、采用改良的线栓法,建立大鼠大脑中动脉栓塞(MCAO)的脑缺血再灌注损伤动物模型,观察EFBHD对局灶性脑缺血再灌注损伤的保护作用。